| 货号 | HCA235 |
| 克隆号 | AbD20669_hIgG1 |
| 同种亚型 | IgG1 |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Human anti omalizumab specificity ELISA A microtiter plate was coated over night with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti omalizumab (clone AbD20669_hIgG1) at a concentration of 2 µg/ml and QuantaBlu™ fluorogenic peroxidase substrate | |
Human anti omalizumab specificity titration ELISA A microtiter plate was coated over night with human IgG1/kappa, human IgG1/lambda, trastuzumab or omalizumab at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti omalizumab (clone AbD20669_hIgG1) titrated to the given concentrations in PBST+10% human serum, followed by QuantaBlu™ fluorogenic peroxidase substrate | |
Omalizumab bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated over night with Human anti omalizumab (clone AbD20669, HCA236) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of omalizumab. Detection was performed using HRP-conjugated Human anti omalizumab (clone AbD20669_hIgG1) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Human anti omalizumab bridging ELISA for Anti-Drug Antibody (ADA) assay development A microtitre plate was coated over night with omalizumab at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, PBST with (black line) or without (red line) 10% human serum was added spiked with increasing concentrations of Human anti omalizumab (clone AbD20669_hIgG1). Detection was performed using HRP-conjugated omalizumab. The addition of serum influences the binding curve of the assay due to interference from endogenous IgE |
