DARPP-32 Pure 15/DARPP-32 50ug
货号：
611520 基本售价：
4140.0 元 规格：
50ug
产品信息
概述| 货号 | 611520 |
| 描述 | Dopaminergic signaling in midbrain neurons is essential to multiple brain functions and involves the activation of dopamine receptors, such as D1 and D2, which regulate the phosphorylation state of DARPP-32 (dopamine and cyclic AMP-regulatedphospho-protein of Mr = 32,000). D1 receptor ligation causes activation of PKA and phosphorylation of DARPP-32 at Thr-34, which converts DARPP-32 to a potent inhibitor of protein phosphatase 1 (PP1). In addition, DARPP-32 is converted to an inhibitor of PKA via phosphorylation at Thr-75 by cyclin-dependent kinase 5 (Cdk5). D2 receptor ligation inhibits PKA and activates protein phosphatase 2B/calcineurin causing dephosphorylation of DARPP-32. The major function of DARPP-32 may be to inhibit the activity of PP1, which controls the phosphorylation state of neurotransmitter receptors, ion channels, ion pumps, and transcription factors. DARPP-32 -/- mice are defective in the physiologicial and behavioral responses to dopamine. Thus, DARPP-32, a bifunctional signal transduction molecule that differentially controls a Ser/Thr kinase and a Ser/Thr phosphatase, is a critical element of dopaminergic neurotransmission and normal brain function. |
| 同种亚型 | IgG1 |
| 反应种属 | Rat (QC Testing) Mouse (Tested in Development) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IF |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human DARPP-32 aa. 70-181 |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.
|
| 存储溶液 | Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. |
| 浓度 | 250 µg/ml |
