货号 | IC6875R-100 |
简介 | Recombinant Monoclonal Antibody |
别名 | HA Epitope Tag; HA Tag; HA; Hemagglutinin |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Stains N-terminal,C-terminal, and Internal SequenceHA-taggedprotein transfectants but not irrelevant transfectants in flow cytometry. |
使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | ABB51961.1 (Viral) |
纯化方式 | Protein A or G purified from cell culture supernatant |
免疫原 | HA tag peptide Accession # ABB51961 |
标记 | Alexa Fluor 647 |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of HA Tag in HEK293 Human Cell Line Transfected withHA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) HA-tagged proteins (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Mouse Anti-HATag Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC6875R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |