| 货号 | MCA2334A488 |
| 克隆号 | CC327 |
| 同种亚型 | IgG2b |
| 反应种属 | Bovine |
| 来源宿主 | Mouse |
| 应用 | F* |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Phycoerythrin conjugated Mouse anti Bovine TNF alpha antibody, clone CC327 used for the evaluation of TNF-α expression by bovine PBMC following M. bovisinfection. Image caption: Identification of multifunctional IFN-γ, IL-2 and TNF-α CD4+ cells in natural M. bovis infection. PBMC from naturally M. bovis infected cattle were cultured in the presence of either PPD-B or medium and the co-expression of IFN-γ, IL-2 and TNF-α determined by ICS flow cytometry. Histograms were gated on singlet, live lymphocytes and then all CD4+ cells analysed for all combinations of cytokine productivity. The upper histograms show the total proportion of CD4+ cells staining for expression of IFN-γ, IL-2 or TNF-α following stimulation with PPD-B (left panel) or medium control (right panel). Subgating of the IFN-γ+ and IFN-γ- CD4+ cells provides histograms that represent all possible functionalities for the expressing of the 3 cytokines, as shown in the lower histograms. The numbers indicate percentage of CD4+ cells and data are representative of 1 of 10 naturally infected cattle. From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194. | |
Published customer image: Mouse anti Bovine TNF alpha antibody, clone CC327 (MCA2334) used as a capture reagent for the evaluation of TNF production using a sandwich ELISA with biotinylated clone CC328 (MCA2335B) as a detection reagent. Image caption: Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-?-) and S. simulans (-?-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL. From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulansandS. epidermidis.Vet Res. 2011 Mar 17;42:49. | |
Published customer image: Mouse anti Bovine TNF alpha antibody, clone CC327 (MCA2334) used as a capture reagent for the evaluation of TNF production using a sandwich ELISA with biotinylated clone CC328 (MCA2335B) as a detection reagent. Image caption: Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-?-) and spontaneously eliminated (-¦-) S. epidermidisandS. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL. From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulansandS. epidermidis.Vet Res. 2011 Mar 17;42:49. |
