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货号： bs-0596R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0596R
- 英文名称
- Dopamine beta Hydroxylase
- 中文名称
- 多巴胺β羟化酶抗体
- 别 名
- DBH; DBM; Dopamine beta hydroxylase; Dopamine-Beta-Hydroxylase; Dopamine beta monooxygenase; DOPO_RAT; DOPO_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 神经生物学 合成与降解
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 70kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human DBH:471-550/621
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Dopamine-beta-Hydroxylase (DBH), Dopamine can be transformed into Noradrenalin. Conversion of dopamine to noradrenaline. [SUBUNIT] Homotetramer composed of two non-covalently bound disulfide-linked dimers. [SUBCELLULAR LOCATION] Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (Potential). Belongs to the copper type II ascorbate-dependent monooxygenase family.
Function:
Conversion of dopamine to noradrenaline.
Subunit:
Homotetramer composed of two non-covalently bound disulfide-linked dimers (By similarity).
Subcellular Location:
Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen (By similarity).
Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (By similarity).
Tissue Specificity:
Chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system.
Similarity:
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain.
SWISS:
P09172
Gene ID:
1621
Database links:Entrez Gene: 280758 Cow
Entrez Gene: 1621 Human
Entrez Gene: 13166 Mouse
Entrez Gene: 25699 Rat
Omim: 223360 Human
Omim: 609312 Human
SwissProt: P15101 Cow
SwissProt: P09172 Human
SwissProt: Q64237 Mouse
SwissProt: Q05754 Rat
Unigene: 223858 Human
Unigene: 591890 Human
Unigene: 167781 Mouse
Unigene: 87166 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Dopamine-β-Hydroxylase（DBH）—多巴胺β羟化酶。可以将多巴胺转化成去甲肾上腺素。是肾上腺素能神经元的标志。
- 产品图片
Sample:
Jurkat (Human) Cell Lysate at 40 ug
Primary: Anti-Dopamine beta Hydroxylase (bs-0596R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOPO) Polyclonal Antibody, Unconjugated (bs-0596R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.