货号 | AHP1032 |
同种亚型 | Polyclonal IgG |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | E, FN, P*, WB |
供应商 | Bio-Rad Antibodies |
溶解方法 | Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
运输条件 | |
存放说明 | Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human interleukin-2 (AHP1032) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) | |
Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human interleukin-2 (AHP1032) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) | |
Sandwich ELISA analysis of human interleukin-2 using Rat anti Human IL-2 (MCA1553) as a capture reagent and biotinylated Rabbit anti Human IL-2 (AHP1032B<) as a detection reagent with recombinant human IL-2 (PHP042) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected |