| 货号 | HCA208 |
| 克隆号 | 17829 |
| 同种亚型 | HuCAL Fab monovalent |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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ELISA demonstrating the specificity of HCA208 using various antigens as coating reagents (A: Human serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Bevacizumab, E: Adalimumab, F: Alemtuzumab, G: Infliximab, H: Ustekinumab). Antigens were coated at 5 µg/mL on a microtiter plate overnight. After washing and blocking with 5% BSA, the anti-Ustekinumab antibody AbD17829 (20µL from a 2µg/mL solution) was added followed by Mouse anti-V5-tag:HRP (MCA1360P)in HiSPEC buffer (BUF049). Detection was performed using QuantaBlu™ fluorogenic peroxidase substrate | |
Inhibition of Ustekinumab binding to IL-23 by HCA208. IL-23 (1.0 µg/mL) was used as the coating antigen followed by a pre-incubated mixture of the anti-idiotypic antibody HCA208 titrated into a constant amount of Ustekinumab (0.3 µg/mL). Free Ustekinumab still capable of binding to the plate was detected using Mouse anti Human IgG(Fc):HRP (MCA647P). Data is shown as the mean of three measurements | |
HCA208 PK assay - bridging format. Anti-Ustekinumab antibody HCA208 (AbD17829) was coated at 1.0 µg/mL on a microtiter plate overnight. After washing and blocking with 5% BSA in PBST, Ustekinumab spiked into 10% human serum was added. Detection was performed by adding HRP conjugated anti-Ustekinumab antibody (HCA210), 2 µg/mL in HiSPEC buffer (BUF049), plus QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements |
