| 货号 | HCA236 |
| 克隆号 | AbD20669 |
| 同种亚型 | HuCAL Fab monovalent |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Human anti omalizumab specificity ELISA A microtiter plate was coated over night with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti omalizumab (clone AbD20669) at a concentration of 2 µg/ml, followed by an HRP-labelled anti-His tag in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Human anti omalizumab specificity titration ELISA A microtiter plate was coated over night with Human IgG1/kappa, Human IgG1/lambda, trastuzumab or omalizumab at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti omalizumab (clone AbD20669) titrated to the given concentrations in PBST, followed by an HRP-labelled anti-His tag in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Omalizumab bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated over night with Human anti omalizumab (clone AbD20669) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of omalizumab. Detection was performed using HRP-conjugated Human anti omalizumab (clone AbD20669) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit® | |
Omalizumab bridging ELISA for Pharmacokinetic (PK) assay development A microtiter plate was coated over night with Human anti omalizumab (clone AbD20669) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of omalizumab. Detection was performed using HRP-conjugated Human anti omalizumab (clone AbD20669_hIgG1, HCA235) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit® | |
Human anti omalizumab inhibits the binding of omalizumab to IgE A microtiter plate was coated over night with Human IgE (HCA171) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of omalizumab (0.3 µg/ml) plus increasing concentrations of Human anti omalizumab (clone AbD20669) was added. Free omalizumab, still capable of binding to the IgE-coated plate, was detected using Goat anti Human IgG (Fc):HRP antibody and QuantaBlu™ fluorogenic peroxidase substrate |
