| 货号 | HCA212 |
| 克隆号 | AbD17837 |
| 同种亚型 | HuCAL Fab monovalent |
| 来源宿主 | HuCAL |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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HCA212 Specificity ELISA To confirm antibody specificity, a microtiter plate was coated overnight with various antigens at a concentration of 5µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HCA212 at 2µg/ml, followed by Mouse anti Strep-tag classic:HRP (MCA2489P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
HCA212 Specificity Titration ELISA A microtiter plate was coated overnight with Human IgG1/kappa, Human IgG1/lambda or Infliximab at a concentration of 5µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HCA212 titrated to the given concentrations in PBST+10% human serum, followed by Mouse anti Strep-tag classic:HRP (MCA2489P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
HCA212 Sensitivity ELISA To assess antibody sensitivity, a microtiter plate was coated overnight with HCA212 at 5µg/ml. After washing and blocking with PBST+5% BSA, Infliximab was added at the given concentrations. Detection was performed using Mouse anti Human IgG (Fc) CH2 domain:HRP (MCA647P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Infliximab Inhibition ELISA To assess the ability of HCA212 to inhibit the binding of Infliximab to its target molecule, a microtiter plate was coated overnight with TNFα at a concentration of 1.0µg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of HCA212 titrated into a constant amount of Infliximab (IFX, 0.3µg/ml) was added. Free Infliximab, still capable of binding to the plate, was detected using Mouse anti Human IgG (Fc) CH2 domain:HRP (MCA647P) | |
Infliximab Bridging ELISA for Pharmacokinetic (PK) Assay Development A microtiter plate was coated overnight with HCA212 at a concentration of 1µg/ml. After washing and blocking with PBST+5% BSA, Infliximab spiked in 10% human serum was added at the given concentrations. Detection was performed using HRP-conjugated HCA213 at 2µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate | |
Infliximab Bridging ELISA for Pharmacokinetic (PK) Assay Development A microtiter plate was coated overnight with HCA212 at a concentration of 5µg/ml. After washing and blocking with PBST+5% BSA, Infliximab spiked in 10% Human serum was added at the given concentrations. Detection was performed using HRP-conjugated HCA215 at 2µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate |
