| 货号 | MCA1783 |
| 克隆号 | CC302 |
| 同种亚型 | IgG1 |
| 反应种属 | Bovine |
| 来源宿主 | Mouse |
| 应用 | E, F* |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Reconstitute with 1 ml distilled water |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Store at +4oC. DO NOT FREEZE This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer’s Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time. From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293. | |
Published customer image: Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 µg/ml polyI:C, 0.5 µg/ml R-848 or 5 µg/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005. From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705. | |
Published customer image: γδ T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5×104 CFUs of B. abortus 2308, and two weeks later γδ T cells (>95% purity) and an enriched TCRαβ (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean ± SD is shown; * P<0.05 versus the enriched TCRαβ cells. Results are representative of two independent experiments. From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine ?d T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978. | |
Published customer image: γδ T cells require TNF-α to protect against B. abortus infection. C57BL/6 mice treated with anti-TCRγδ mAb or hamster IgG on day -1 and day 3 post-infection with 5×104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-α on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean ± SEM of 10 mice/group is depicted; *P<0.05 versus hamster IgG-treated C57BL/6 mice. Results are from two independent experiments. C. and D. Splenocytes (5×106/ml) from 4 or 7 day B. abortus-infected mice depleted or not of γδ T cells and/or TNF-a or splenocytes from naïve C57BL/6 mice were left unstimulated and cultured for 3 days at 37°C/5%CO2; supernatants were harvested for C. TNF-α- or D. IFN-γ- specific ELISA. The mean ± SD of triplicate wells is shown. The results from 7 dpi are representative of two independent experiments. NS = not significant. ND = cytokine production from uninfected mice neutralized of γδ T cells was not determined. * P<0.05 as compared to mice not depleted of γδ T cells within the same TNF-α treatment group at the same time point. † P<0.05 as compared to C57BL/6 mice treated with IgG only at the same time point. E. The median fluorescence intensity (MFI) of CD69 expression by splenic NK and γδ T cells as measured by flow cytometry is shown for naïve C57BL/6 and B. abortus-infected (after 4 days of infection with 5×104 CFUs of strain 2308) C57BL/6 mice. Data depict the mean ± SD from 5 mice/group; *P<0.05 versus naïve mice and † P<0.05 versus B. abortus-infected mice not neutralized of their TNF-α. F. and G. Peritoneal macrophages from C57BL/6 and TCRδ-/- mice were infected with B. abortus (30 bacteria:1 macrophage), and F. TNF-α levels in supernatants and G. intracellular colonization were measured. Data represent the mean ± SD of triplicate wells/group. From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortusInfections.PLoS ONE 6(7): e21978. | |
Published customer image: Bovine γδ T cells impair B. abortus replication in autologous macrophages via IFN-γ. A.–F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous γδ T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P<0.05 versus colonization by cultured macrophages only from the same animal. B.,D.,F. IFN-γ levels were determined in cell culture supernatants. At 72 and 120 h post-infection, macrophage plus γδ T cell co-cultures from each calf contained more IFN-γ than from macrophages cultured alone. G. NK1.1+ cell-depleted Rag-1-/- mice (4–5 per group) received PBS, bovine macrophages (5×105/mouse), bovine macrophages (5×105/mouse) plus autologous γδ T cells (1×107/mouse), or bovine macrophages plus autologous CD4+ T cells (1×107/mouse) i.p. one day prior to infection with 1×104 CFUs of B. abortus. All cells were derived from Calf #1. On day 7, splenic colonization was determined. Depicted is the mean ± SD; *P<0.05 versus mice given PBS or macrophages only. From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortusInfections.PLoS ONE 6(7): e21978. | |
Published customer image: B. abortus infection does not induce IL-17 or IFN-γ production by γδ T cells. A. Splenocytes from naïve or B. abortus-infected mice (7 dpi) were stimulated overnight with PMA/Ionomycin and brefeldin A was added for the last 3 h of culture. Following surface staining, cells were permeabilized and stained for intracellular IL-17 or IFN-?. Top panel, the proportion of IL-17 producing γδ T cells was determined following gating on lymphocytes. Second panel from top, cells were gated on CD4+ (CD3+) T cells and assayed for IL-17 production. Third panel from top, cells were gated on γδ T cells (CD3+/TCR γδ+) and assayed for IFN-γ production. Bottom panel, cells were gated on CD4+ (CD3+) T cells and assayed for IFN-γ production. Depicted is the mean ± SD of 5 mice/group and is representative of two independent experiments. B. γδ T cells were sorted from naïve or B. abortus-infected (7 dpi) mice and stimulated for 72 h with PMA/Ionomycin. Cytokine levels in supernatant were determined by ELISA. Depicted is the mean ± SD of triplicate wells. *P<0.05 versus cytokine production by ?d T cells from naïve mice. From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortusInfections.PLoS ONE 6(7): e21978. | |
Published customer image: Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN-γ within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN-γ and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments. From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194. | |
Published customer image: Mouse anti Bovine interferonγ antibody, clone CC302 used for the identification of interferonγ expressing dog lymphocytes by flow cytometry. Image caption: Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes. From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92. | |
Published customer image: Mouse anti bovine interferon gamma antibody, clone CC302 used for the measurement of the gamma interferon response to M. avium infection in a caprine model by ELISA in conjunction with biotinylated Mouse anti Bovine interferon γ antibody, clone CC330 as a capture reagent. Image caption: Time course and intensity of MAP-specific antibody response (A) and antigen-induced (Johnin, 4 µg/mL) IFN-? response (B) of inoculated and control goats. Box and Whisker Plot represents median value, 25% and 75% percentiles (box), range, outlier values (?), and extreme values (*). Different letters indicate significant differences between groups (Mann–Whitney-U test, P?=?0.05): a – V1 vs. V2, b – V1 vs. V3, c – V1 vs. V4, d – V2 vs. V3, e – V2 vs. V4, f – V3 vs. V4. From: Köhler H, Soschinka A, Meyer M, Kather A, Reinhold P, Liebler-Tenorio E. Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection. BMC Vet Res. 2015 Mar 24;11(1):74. | |
Published customer image: Mouse antiBovine Ifn γantibody, clone CC302 (MCA1783) and Mouse anti Bovine IL-4 antibody, clone CC303 (MCA1820) used to evaluate cytokine expression by canine peripheral blood cells following short term in vitro stimulation and culture by flow cytometry. Image caption: Internal controls to validate the cross-reactivity of anti-human cytokine mAbs with canine cytokines following short-term whole blood cultures in vitro. Non-specific binding was monitored by using isotype matched reagents (top panels). Blocking strategy with host serum was also applied (bottom panels). Controls of reference cross-reactive mAbs (anti-bovina IFN-? and IL-4) were also included. Dot plot distribution graphs of empty channel (FL4 fluorescence) versus PE-emission channel (FL2) were used to quantify the percentage of cytokine+ lymphocytes in unstimulated controls (left panels) and PMA + LPS stimulated cultures (right panels). Only Q1 and Q4 quadrants are represented. Gray background highlights the reactivity above 0.5 % in the quadrant Q1 From: Moreira ML, Dorneles EM, Soares RP, Magalhães CP, Costa-Pereira C, Lage AP, Teixeira-Carvalho A, Martins-Filho OA, Araújo MS. Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining. Acta Vet Scand. 2015 Sep 11;57:51. | |
Published customer image: RPE conjugated mouse anti Bovine Ifn-γ antibody, clone CC302 used to evaluate ifn-γ expression in canine lymphocytes by flow cytometry. Image caption: CD4+ T cells from Ech_0660 mutant vaccinated and wild-type E. chaffeensis infected animals produce IFNγ in response to E. chaffeensis antigen. PBMC from dogs vaccinated with the Ech_0660 mutant and challenged with wild-type E. chaffeensis (groups 1–3, as in Fig 2) were cultured for 5 days at 4x106 cells/mL in the presence or absence of 10 μg/mL E. chaffeensis host-cell free lysate grown in the tick ISE6 cell line. On day 5, brefeldin A was added for the last 6 hours of culture. CD4+ T cells were stained for intracellular expression of IFNγ and analyzed by flow cytometry. (A) Representative flow plots from animals in groups 1, 2 and 3, gated on total live cells and total CD3+CD4+ T cells. (B) The percentage of IFNγ+ cells of total CD4+ T cells in the blood measured over the course of the experiment. Background (mock stimulated) IFNγ production was subtracted, and results represent change over mock. From: McGill JL, Nair ADS, Cheng C, Rusk RA, Jaworski DC, Ganta RR (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host. PLoS ONE 11(2): e0148229. | |
Published customer image: RPE conjugated mouse anti Bovine Ifn-γ antibody, clone CC302 used to evaluate ifn-γ expression in canine lymphocytes by flow cytometry. Image caption: CD8+ T cells from Ech_0660 vaccinated and wild-type E. chaffeensis infected animals proliferate and produce IFNγ in response to E. chaffeensis antigen. CD8+ T cell proliferation and IFNγ production were measured using similar approaches as in Figs 2 and 3. PBMC from dogs in groups 1–3 were cultured for 5 days at 4x106 cells/mL in the presence or absence of 10 μg/mL E. chaffeensis host-cell free lysate. On day 5 of culture, CD8+ T cells were analyzed by flow cytometry for (A) proliferation as measured by Cell Trace Violet dilution; and (B) intracellular production of IFNγ. The frequencies of responding CD8+ T cells were measured over the course of the experiment. Results were gated on total live cells and total CD3+CD8+ T cells. Background (mock stimulated) proliferation or IFNγ production was subtracted and results represent change over mock. From: McGill JL, Nair ADS, Cheng C, Rusk RA, Jaworski DC, Ganta RR (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host. PLoS ONE 11(2): e0148229. |
