货号 | MCA2311GA |
克隆号 | 2A10/11 |
同种亚型 | IgG1 |
反应种属 | Pig |
来源宿主 | Mouse |
应用 | C, F, IF, IP, WB* |
供应商 | Bio-Rad Antibodies |
溶解方法 | Reconstitute with 1.0ml distilled water |
运输条件 | |
存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 (MCA2311GA) | |
Staining of porcine peripheral blood mononuclear cells with Mouse anti Porcine CD163 : FITC (MCA2311F) | |
Published customer image:Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203ahi leukocytes (d) and marked with blue color. Monocytes were gated as CD203alow/- SWC8-(e) CD172ahi(f) leukocytes where the CD203alow/- region was defined as the complementary region to the CD203ahi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR–, red: SLA-DR+) in various body compartments of control and APP-infected pigs. From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98. | |
Published customer image:Representative pictures of immunohistochemical detection of CD163+ cells in tracheobronchial lymph node and spleen. CD163+ cells were detected in tracheobronchial lymph nodes from control (A) and APP-infected pigs (B) and in spleen from control (C) and APP-infected pigs (D). Immunohistochemical visualization: horseradish peroxidase, brown substrate, hematoxylin counterstain; c, cortex; ca, central artery; e, ellipsoid; f, follicle; mz, marginal zone; pals, periarterial lymphatic sheath, rp, red pulp; s, subcapsular sinus. From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98. | |
Published customer image: Mouse anti Pig CD163 antibody, clone 2A10/11 used for the detection of CD163 expressing cells in porcine synovium by immunohistochemistry on cryosections. Image caption: Light microscopy images of CD163 antibody stained histological sections obtained from the synovium (A, C, F, I, L), ligament (B, D, G, J, M), and provisional scaffold (E, H, K, N) at days 0 (A,B), 1 (C, D, E), 5 (F, G, H), 9 (I, J, K), and 14 (L, M, N) following ACL injury. Scale bar represents 50 μm. From: Haslauer CM, Proffen BL, Johnson VM, Hill A, Murray MM. Gene expression of catabolic inflammatory cytokines peak before anabolic inflammatory cytokines after ACL injury in a preclinical model. J Inflamm (Lond). 2014 Nov 1;11(1):34. | |
Published customer image: Mouse anti Porcine CD163 antibody, clone 2A10/11 (MCA2311GA) used for the evaluation of CD163 expression on Sn-CD163 transfected PK15 cells by immunofluorescence. Image caption: Construction of CHOSn-CD163 and PK15Sn-CD163 cell lines. A) Schematic representation of the construction of CHOSn-CD163 and PK15Sn-CD163 cell lines To construct a cell line co-expressing Sn and CD163, CHO-K1 or PK15 cells were transfected with a plasmid containing the Sn cDNA and a geneticine resistance gene. The cells were single cell cloned and clones were screened for Sn expressing cells. After selection for geneticine resistance, the obtained CHOSn or PK15Sn cells were transfected with a plasmid containing the CD163 cDNA and a zeocin resistance gene, which allowed selection of cells expressing both Sn and CD163. B) Immunofluorescence staining of the obtained CHOSn-CD163 or PK15Sn-CD163 cells for Sn and CD163. Some CHOSn-CD163 clones (IF3, IC5 and ID9) and PK15Sn-CD163 clones (IXA3 and IXH7) are represented with their Sn and CD163 expression. From: Delrue I, Van Gorp H, Van Doorsselaere J, Delputte PL, Nauwynck HJ. Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163. BMC Biotechnol. 2010 Jun 29;10:48. | |
Figure A. FITC conjugated mouse anti porcine CD45RA (MCA1751F) and PE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD45RA (MCA1751F) and PE conjugated mouse anti porcine CD163 (MCA2311PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells. | |
Figure A. PE conjugated mouse anti porcine CD163 (MCA2311PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated mouse anti porcine CD163 (MCA2311PE) and FITC conjugated mouse anti porcine CD45RA (MCA1751F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells. |