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货号： bs-0539R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0539R
- 英文名称
- Chromogranin A
- 中文名称
- 嗜铬粒素A抗体
- 别 名
- beta Granin; CGA; CHG A; CHGA; Chromogranin A parathyroid secretory protein 1; Chromogranin A precursor; ChromograninA; Pancreastatin; Parastatin; Parathyroid secretory protein 1; Pituitary secretory protein I; SP I; SP1; SPI; Vasostatin; ER-37; CMGA_HUMAN.
- Specific References (6) | bs-0539R has been referenced in 6 publications.[IF=5.70] Wang, Yuli, et al. "In vitro generation of colonic epithelium from primary cells guided by microstructures." Lab on a Chip (2014). Mouse.PubMed:24647645[IF=4.25] Liu, Yanchun, et al. "Enriched Intestinal Stem Cell Seeding Improves the Architecture of Tissue Engineered Intestine." Tissue Engineering (2014). IHC-P ; Rat.PubMed:25603285[IF=4.25] Liu, Yanchun, et al. "Enriched Intestinal Stem Cell Seeding Improves the Architecture of Tissue Engineered Intestine." Tissue Engineering (2014). IHC-P ; Rat.PubMed:25603285[IF=3.26] Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016). IHC-P ; Chicken.PubMed:27215404[IF=5.70] Wang, Yuli, et al. "Capture and 3D culture of colonic crypts and colonoids in a microarray platform." Lab on a Chip 13.23 (2013): 4625-4634. IF(IHC-F) ; Mouse.PubMed:24113577[IF=8.39] Liu, Yanchun, et al. "HB-EGF Embedded in PGA/PLLA Scaffolds via Subcritical CO 2 Augments the Production of Tissue Engineered Intestine."Biomaterials (2016). IF(IHC-P) ; Rat.PubMed:27380441
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 48kDa
- 细胞定位
- 细胞浆 细胞膜 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CGA:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008].
Function:
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
Subunit:
Interacts with SCG3.
Subcellular Location:
Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.
Post-translational modifications:
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Similarity:
Belongs to the chromogranin/secretogranin proteinfamily.
SWISS:
P10645
Gene ID:
1113
Database links:Entrez Gene: 1113 Human
Entrez Gene: 12652 Mouse
Omim: 118910 Human
SwissProt: P10645 Human
SwissProt: P26339 Mouse
Unigene: 150793 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
嗜铬粒素A蛋白存在与很多组织中：胃肠道内分泌细胞和类癌、甲状旁腺、垂体前叶、肾上腺髓质、神经节、副神经节、胰岛、甲状腺C细胞等组织和其来源的肿瘤都表达该成分.嗜铬素A（CgA）属于可溶性的酸性蛋白，主要存在于神经内分泌细胞的分泌颗粒中，也存在于含有分泌颗粒的内分泌细胞和神经内分泌细胞来源的肿瘤细胞中。检测循环中的免疫反应CgA对神经内分泌瘤具有诊断意义。另外，CgA的免疫组织化学检测对鉴定来源于神经内分泌的肿瘤起到辅助作用。
- 产品图片
Sample:Cerebrum(Mouse)Lysate at 40 ug
Primary: Anti-Chromogranin A(bs-0539R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 48kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CGA/Chromogranin A Polyclonal Antibody, Unconjugated(bs-0539R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining