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货号： bs-1192R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1192R
- 英文名称
- CD14
- 中文名称
- 内毒素受体抗体
- 别 名
- CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_MOUSE; Myeloid cell-specific leucine-rich glycoprotein.
- Specific References (6) | bs-1192R has been referenced in 6 publications.[IF=3.48] Chen, Xiaoming, et al. " Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-κB) and induces cytokine production via Toll-like receptors." Carbohydrate Polymers (2014). other ; Rat.PubMed:24708959[IF=7.81] Chen, Yen‐Ta, et al. "Melatonin Treatment further Improves Adipose‐Derived Mesenchymal Stem Cell Therapy for Acute Interstitial Cystitis in Rat." Journal of Pineal Research (2014). IHC-P ; Rat.PubMed:25132326[IF=2.14] Nau, Christoph, et al. "Alteration of Masquelets induced membrane characteristics by different kinds of antibiotic enriched bone cement in a critical size defect model in the rats femur." Injury (2015). IHC-P ; Rat.PubMed:26652225[IF=1.58] Sun, Bing, et al. "Tight junction proteins and gap junction proteins play important roles in high fat dietary atherosclerosis pathogenesis." Int J Clin Exp Pathol 9.8 (2016): 7969-7976. IHC-P ; Rat.PubMed:0[IF=0.52] "Utomo, Pamudji, et al. ""Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury."" International Journal of ChemTech Research. " IF(ICC) ; Rat.PubMed:0[IF=3.15] Sun, Cheuk-Kwan, et al. "Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury." Am J Transl Res 9.4 (2017): 1543-1560. IHC-P ; Rat.PubMed:28469765
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 干细胞 细胞表面分子 细胞类型标志物 淋巴细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 35/40kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse CD14:201-300/366
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Function:
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Subunit:
Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Similarity:
Contains 11 LRR (leucine-rich) repeats.
SWISS:
P10810
Gene ID:
12475
Database links:Entrez Gene: 929 Human
Entrez Gene: 12475Mouse
Omim: 158120 Human
SwissProt: P08571 Human
Unigene: 163867 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD14又称内毒素受体，属细胞表面糖蛋白家族成员之一。在机体免疫、防御系统引起的一系列反应中介导细胞识别LPS并引起细胞酪氨酸磷酸化、核因子NF-κB转位、触发细胞因子释放和氧自由基产生。CD14在细胞因子产生的调控方面起重要作用,因而对于CD14结构、功能及其临床意义的研究日益受到重视。
- 产品图片
Protein: mouse lung lysate;
Primary: rabbit Anti-CD14(bs-1192R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 40 kD
Observed band size: 40 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD14 Polyclonal Antibody, Unconjugated(bs-1192R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining