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货号： bs-0527R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0527R
- 英文名称
- CD10
- 中文名称
- CD10抗体
- 别 名
- Atriopeptidase; CALLA; CD 10; CD10; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; gp100; Membrane metallo endopeptidase; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; Neprilysin; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP_HUMAN; Neprilysin; Skin fibroblast elastase; SFE.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 干细胞 细胞表面分子 细胞类型标志物 淋巴细胞 b-淋巴细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg /test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 82kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CD10:601-600/750 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5 untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.
Function:
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Subcellular Location:
Cell membrane; Single-pass type II membrane protein.
Post-translational modifications:
Myristoylation is a determinant of membrane targeting.
Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.
Similarity:
Belongs to the peptidase M13 family.
SWISS:
P08473
Gene ID:
4311
Database links:Entrez Gene: 4311 Human
Entrez Gene: 17380 Mouse
Entrez Gene: 24590 Rat
Omim: 120520 Human
SwissProt: P08473 Human
SwissProt: Q61391 Mouse
SwissProt: P07861 Rat
Unigene: 307734 Human
Unigene: 296022 Mouse
Unigene: 33598 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD10即共同型急性淋巴细胞白血病抗原,是II型跨膜糖蛋白.CD10表达于早期B淋巴细胞,某些粒性白细胞,骨髓基质细胞,部分上皮组织及其起源的肿瘤细胞中也有表达.主要应用于某些恶性淋巴瘤和白血病的分型.CD10是一种94kD的锌依赖性细胞膜金属结合蛋白，参与细胞神经肽和激素肽的后分泌过程。它广泛地分布于肾、肝、小肠、胎盘、脉络膜丛、脑腺、腺皮质和白细胞，CD10是生发中心细胞及所致淋巴瘤的标志物。
- 产品图片
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD10 (bs-0527R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 82 kD
Observed band size: 82 kD
Blank control: A549 (blue).
Primary Antibody:Rabbit Anti- CD10 antibody(bs-0527R), Dilution: 3μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0527R, 3μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.