Anti-F-Actin【科瑞奥博】

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Anti-F-Actin

货号： bs-1571R 基本售价： 580.0 元规格： 50ul

规格：50ul: 价格：580.00元

规格：100ul: 价格：920.00元

规格：200ul: 价格：1600.00元

产品信息

产品编号: bs-1571R

英文名称: F-Actin

中文名称: 纤维状肌动蛋白抗体

别名: f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1.

: Specific References  (14)     |     bs-1571R has been referenced in 14 publications.
[IF=3.73] Su, Bao, et al. "In Vitro and In Vivo Evaluations of Nano-Hydroxyapatite/Polyamide 66/Glass Fibre (n-HA/PA66/GF) as a Novel Bioactive Bone Screw." PLOS ONE 8.7 (2013): e68342.  Mouse.
PubMed:23861888
[IF=3.73] Zhong, Weiliang, et al. "Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds." PLOS ONE 8.4 (2013): e61283.  Rat.
PubMed:23637803
[IF=3.22] Zhong, Weiliang, et al. "An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus."Microfluidics and Nanofluidics: 1-11.
PubMed:N/A
[IF=2.80] Kang, Hongyan, et al. "A Potential Gravity-Sensing Role of Vascular Smooth Muscle Cell Glycocalyx in Altered Gravitational Stimulation." Astrobiology (2013).  WB ;  Rat.
PubMed:23848471
[IF=2.55] Zhao, Ran, et al. "Hepatoma cell line HepG2. 2.15 demonstrates distinct biological features compared with parental HepG2." World journal of gastroenterology: WJG 17.9 (2011): 1152.  WB ;  Human.
PubMed:21448419
[IF=0.86] Xie, Chaoming, Xiong Lu, and Kefeng Wang. "Pulse Electrochemical Synthesis of Spherical Hydroxyapatite and Silver Nanoparticles Mediated by the Polymerization of Polypyrrole on Metallic Implants for Biomedical Applications." Particle & Particle Systems Characterization (2015).  Rat.
PubMed:not posted yet
[IF=19.68] Yuan, Lin, et al. "Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis." Nature Cell Biology (2015).  IHC-P ;  Mouse.
PubMed:26280536
[IF=0.00] Deguchi, Takahiro. "Development of 3D super-resolution tomographic STED microscopy and its application to studies on bone resorption." (2015).  other ;
PubMed:000
[IF=2.52] Chen, S. M., et al. "Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans." Andrology (2016).  WB ;  Human.
PubMed:27369112
[IF=1.86] Siar, Chong Huat, et al. "Invadopodia proteins, cortactin, N‐WASP and WIP differentially promote local invasiveness in ameloblastoma." Journal of Oral Pathology & Medicine (2016).  IHC-P ;  Human.
PubMed:26752341
[IF=0.18] Du, Ailin, et al. "Damage of hippocampal neurons in rats with chronic alcoholism." Neural regeneration research 9.17 (2014): 1610.  IHC-P ;  Rat.
PubMed:25368648
[IF=1.84] Zhang, Pengfei, et al. "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." Theriogenology (2017).  WB ;  Chicken.
PubMed:28460670
[IF=2.22] Li, Haihong, et al. "Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice." Journal of molecular histology 47.5 (2016): 485-490.  IHC-P ;  Mouse.
PubMed:27492422
[IF=1.69] Guo, Xiliang, and Xuezheng Liu. "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." Molecular Medicine Reports. (2017).  IHC-P ;  Rat.
PubMed:28656312

规格价格: 50ul/580元购买 100ul/920元购买 200ul/1600元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 免疫学细胞骨架

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Pig, Cow, Sheep,

产品应用: WB=1:1000-5000 ELISA=1:500-1000 Flow-Cyt=3μg /test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 42kDa

细胞定位: 细胞浆

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit:1-50/374

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins, found in muscletissues, are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin.

SWISS:
P60709

Gene ID:
60

Database links:

Entrez Gene: 60 Human

Entrez Gene: 11461 Mouse

Entrez Gene: 100009272 Rabbit

Entrez Gene: 81822 Rat

Omim: 102630 Human

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P29751 Rabbit

SwissProt: P60711 Rat

Unigene: 520640 Human

Unigene: 708120 Human

Unigene: 727576 Human

Unigene: 328431 Mouse

Unigene: 391967 Mouse

Unigene: 94978 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

纤维状肌动蛋白F-Actin主要存在于横纹肌原肌纤维细丝及平滑肌中，是肌肉运动和细胞运动的重要蛋白。

产品图片: Sample: Lane1:Hela (Human) Whole Cell Lysate at 30 ug
Lane2:Jurkat (Human) Whole Cell Lysate at 30 ug
Lane3:293T (Human) Whole Cell Lysate at 30 ug
Lane4:Hepg2 (Human) Whole Cell Lysate at 30 ug
Lane5:A431 (Human) Whole Cell Lysate at 30 ug
Lane6:A549 (Human) Whole Cell Lysate at 30 ug
Lane7:H9C2 (Rat) Whole Cell Lysate at 30 ug
Lane8:Testis (Mouse) Tissue Lysate at 30 ug
Primary: Anti-F-Actin(bs-1571R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti- F-Actin antibody (bs-1571R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.