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货号： bs-1254R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1254R
- 英文名称
- Apolipoprotein D
- 中文名称
- 载脂蛋白D抗体
- 别 名
- Apolipoprotein D; APOD; APO D; APOD protein; APOD_HUMAN; Apolipoprotein D; Apo-D; ApoD.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 心血管 细胞生物 免疫学 神经生物学 Alzheimers
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 19kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human APOD:81-189/189
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Apolipoprotein D (APO D), a glycoprotein involved in the human plasma lipid transport system. It is a progesterone binding glycoprotein of 24,000 dalton monomer molecular size, is a constituent of high density lipoprotein in plasma. The function of apolipoprotein D in the metabolism of plasma lipoproteins is unclear but the observation that this protein forms complexes with lecithin: cholesterol acyltransferase has led to the suggestion that apolipoprotein D may be involved in cholesterol esterification and transport of substrates and products of the reaction. Apolipoprotein D is expressed in a range of normal tissues including axillary apocrine glands, adrenal cortex and corpus luteum. Peripheral nerves, pituitary, testis, cerebellum and renal tubes are also positive. APO D was first isolated in large quantity as GCDFP 24, the major protein component of most human breast cyst fluids. In recent studies, Apo D immunoreactivity has been shown to be an early indicator of prostate cancer and advanced primary prostate tumors.
Function:
APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
Subunit:
Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.
Post-translational modifications:
N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor).
Similarity:
Belongs to the calycin superfamily. Lipocalin family.
SWISS:
P05090
Gene ID:
347
Database links:Entrez Gene: 347 Human
Omim: 107740 Human
SwissProt: P05090 Human
Unigene: 522555 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
载脂蛋白D(apolipoprotein D,apoD)是一个糖蛋白,它最初是在人血浆的高密度脂蛋白中被分离出来的。apoD在结构上与其他类型的载脂蛋白存在很大的差异,被归入脂肪促成素家族。apoD可以与胆固醇、黄体酮、胆红素等多种疏水性小分子结合。
apoD在多种脊索动物的各类组织中广泛表达, 在脊椎动物中重要的生理功能。最近的研究表明,apoD可以作为多种癌症及神经系统疾病的早期诊断标记, apoD是一个多配体、多功能的蛋白质.
- 产品图片
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Apolipoprotein D (bs-1254R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 30 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOD Polyclonal Antibody, Unconjugated(bs-1254R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining