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货号： bs-23788R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-23788R
- 英文名称
- NG2
- 中文名称
- 黑色素瘤硫酸软骨素蛋白多糖4抗体
- 别 名
- MELCSPG; AN2; AN2 proteoglycan; Chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; CSPG4; Cspg4 chondroitin sulfate proteoglycan 4; CSPG4_HUMAN; HMW-MAA; HSN tumor-specific antigen; Kiaa4232; MCSP; MCSPG; MEL-CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; 4732461B14Rik; MSK16.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 神经生物学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 247kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human NG2:701-800/2322 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
NG2 (also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16 and MEL-CSPG) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (Threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with Alpha 4 Beta1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Microvessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering.
Function:
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Subunit:
Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
Subcellular Location:
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
Tissue Specificity:
Detected only in malignant melanoma cells.
Post-translational modifications:
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
Similarity:
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains.
SWISS:
Q6UVK1
Gene ID:
1464
Database links:Entrez Gene: 1464Human
Entrez Gene: 121021Mouse
Omim: 601172Human
SwissProt: Q6UVK1Human
SwissProt: Q8VHY0Mouse
Unigene: 513044Human
Unigene: 41329Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-NG2 (bs-23788R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 247 kD
Observed band size: 350 kD
Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NG2) Polyclonal Antibody, Unconjugated (bs-23788R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NG2) Polyclonal Antibody, Unconjugated (bs-23788R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.