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货号： bs-20676R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-20676R
- 英文名称
- MMP7
- 中文名称
- 基质金属蛋白酶-7抗体
- 别 名
- Matrilysin; Matrilysin uterine; Matrin; Matrix Metalloproteinase 7; MMP-7; MMP 7; MMP7; MPSL1; PUMP 1; Pump 1 protease; PUMP1; Uterine Matrilysin; Uterine metalloproteinase; MMP7_MOUSE.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 免疫学 神经生物学 细胞凋亡
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Mouse, Rat,
- 产品应用
- WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 30kDa
- 细胞定位
- 细胞外基质 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse MMP7:51-150/264
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
Function:
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Subcellular Location:
Secreted, extracellular space, extracellular matrix (Probable).
Similarity:
Belongs to the peptidase M10A family.
SWISS:
P09237
Gene ID:
17393
Database links:Entrez Gene: 4316 Human
Entrez Gene: 17393 Mouse
Entrez Gene: 25335 Rat
Omim: 178990 Human
SwissProt: P09237 Human
SwissProt: Q10738 Mouse
SwissProt: P50280 Rat
Unigene: 2256 Human
Unigene: 4825 Mouse
Unigene: 10282 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
合成与降解（Synthesis and Degradation）
MMP7属于MMP基因家族的成员之一，MMP-7又称溶基质蛋白素(Matrilysin)，其主要功能为降解粘蛋白、纤维连结蛋白和层粘连蛋白，在肿瘤细胞突破基底膜屏障和浸润、转移中起重要作用。目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)转移、浸润的研究及用于机体各种组织的发育和修复、肿瘤发生发展、炎症反应等过程中作用的研究。
- 产品图片
Sample:
Lymph node(Rat) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-MMP7 (bs-20676R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP7) Polyclonal Antibody, Unconjugated (bs-20676R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP7) Polyclonal Antibody, Unconjugated (bs-20676R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.