| 描述 | TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM®Method tab below. TransAM® AP-1 Transcription Factor ELISA KitsTransAM AP-1 Kits provide everything needed to study activated AP-1, including a positive control extract. Kits are available with antibodies specific for the activated form of c-Fos, FosB, JunD or phosphorylated c-Jun. A family kit is also available with the ability to independently screen the members of the AP-1 family (c-Fos, FosB, Fra-1, c-Jun, JunB & JunD) simultaneously. The Fra-1 and JunB antibodies can be used with human extracts, while the c-Fos, FosB and JunD antibodies can be used with human, mouse and rat extracts. c-Jun phosphorylated at Ser73 and JunD phosphorylated at Ser100 can be detected in human and mouse extracts, as this site is conserved between c-Jun and JunD. Recombinant c-Fos and Recombinant c-Jun proteins are available separately to generate an optional protein standard curve in the TransAM AP-1 Transcription Factor ELISA kits. Click the AP-1 Info tab below for data and more information; kit manuals can be downloaded under the Documentstab. |
