货号 | 41098 |
描述 | TransAM® Flexi Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. The TransAM Flexi format enables you to look at NFκB binding sites of your own design. This kit works by combining a stimulated nuclear extract with biotinylated oligos you generated to capture activated NFκB binding. The binding reaction is then transferred to the wells of a 96-well streptavidin coated plate and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM®Flexi tab below. TransAM® Flexi NFκB Transcription Factor ELISA KitsTransAM Flexi NFκB Kits provide everything needed to study Nuclear Factor κB (NFkB), including a positive control extract and positive control biotinylated oligonucelotide. Kits are available with antibodies specific for the activated form of p50 and p65. A family kit is also available with the ability to screen the members of the NFkB family (p50, p52, p65, c-Rel and RelB). The p50 and p52 antibodies can be used with human and mouse extracts, while the p65 and RelB antibodies can be used with human, mouse and rat extracts. The c-Rel antibody can only be used with human extracts. Recombinant p50 and Recombinant p65 proteins are available separately to generate an optional protein standard curve in the TransAM NFκB Transcription Factor ELISA kits. See the NFkB Info tab below for kit data and more information; the kit manual can be downloaded under the Documentstab. * Technology covered under EAT-filed patents and licensed to Active Motif. Use of TransAM in NFκB-related drug discovery may be covered under U.S. Patent No. 6,150,090 and require a license from Ariad Pharmaceuticals (Cambridge, MA, USA). |
供应商 | Active Motif |
运输条件 | Dry Ice |
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