LightSwitch™ Promoter GoClone®
货号：
32001 基本售价：
7177.5 元 规格：
5 ug
产品信息
概述| 货号 | 32001 |
| 描述 | Our scientists created the LightSwitch Promoter Reporter Collection by using an algorithm to evaluate over 5 million human cDNA sequences in order to identify transcription start sites (TSS) throughout the human genome. Based on these TSS predictions, over 18,000 human promoter sequences of ~1 kb (-900…+100 bp relative to the TSS) were cloned into the pLightSwitch_Prom reporter vector, which utilizes the novel, engineered RenSP luciferase gene. After transfection, luciferase activity is assayed using the optimized regents found in LightSwitch Luciferase Assay Kit. In addition to the over 18K endogenous promoter clones that make up the LightSwitch Promoter Reporter Collection, Active Motif also offers a collection of LightSwitch Synthetic Response Elements. These constructs contain repeats of a transcription factor binding site motif, cloned upstream of a minimal HSV-TK promoter and the RenSP luciferase gene in the Long-range Enhancer Reporter Vector, pLightSwitch_LR vector. This makes them ideal for use as a primary screen to identify compounds or conditions that affect the activity of a biological pathway. Afterward, a secondary screen can be performed using related endogenous promoter reporter constructs to detect subtle differences between compounds or conditions. |
性能| 供应商 | Active Motif |
| 运输条件 | Blue Ice |
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