Investigator Argus X-12 QS (100)【科瑞奥博】

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Investigator Argus X-12 QS (100)

货号： 383225 基本售价： 24000.0 元规格： 100Reactions

产品信息

概述

货号

383225

简介

For multiplex amplification of 12 X-chromosomal STRs, D21S11 and Amelogenin, with an innovative Quality Sensor

描述

Performance

The Investigator Argus X-12 QS Kit utilizes fast-cycling technology to provide reliable results in approximately 80 minutes.
The novel Fast Reaction Mix 2.0 is based on proven QIAGEN Multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results.
The Investigator Argus X-12 QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

Principle

The Investigator Argus X-12 QS Kit uses QIAGENs fast-cycling PCR technology for the direct, simultaneous amplification of 12 X-chromosomal markers, D21S11 and Amelogenin. The amplification of the 12 X-chromosomal markers (see Table 1 and 2), with heightened discrimination power, meets the needs for complex kinship and paternity testing and helps fulfill the demands of complicated deficiency cases involving at least one female. The 12 X-chromosomal markers are clustered into four linkage groups (three markers per group; Table 1), and thus each set of three markers is handled as a haplotype for genotyping.

Table 1. Linkage groups
Linkage group
1 (Xp22)	DXS8378 – DS10135 – DXS10148
2 (Xp11)	DXS7132 – DXS10074 – DXS10079
3 (Xp26)	HPRTB – DXS10101 – DXS10103
4 (Xp28)	DXS7423 – DXS10134 – DXS10146

The innovative Quality Sensors provides additional information on the sample quality and PCR success beyond the profile of your sample. With the Quality Sensor (QS) being amplified as an amplification control, it is possible to determine:

A successful PCR run
A failed PCR run
Lack of DNA in your sample

This additional information will help you analyze your data, and guides you with respect to further sample processing. It will also help you reduce unnecessary PCR re-runs, saving you time and money.
The additional autosomal marker D12S11 acts as alignment marker. Now, you have the possibility to align your X-chromosomal profile from the Investigator Argus X-12 QS Kit with a profile of any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups.

Procedure

The 12 X-chromosomal markers, D21S11 and the gender-specific Amelogenin, are amplified simultaneously in a single PCR run and labeled with one of the dyes listed in the Table 2: "Investigator Argus X-12 QS Kit dyes and corresponding markers".
The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Technical specifications" for more information.

Table 2. Investigator Argus X-12 QS Kit dyes and corresponding markers
Dyes	Markers
6-FAM	QS1	Amelogenin	DXS10103	DXS8378	DXS10101	DXS10134
BTG	DXS10074	DXS7132	DXS10135
BTY	DXS7423	DXS10146	DXS10079
BTR	DXSHPRTB	DXS10148	D21S11

Table 3. Investigator Argus X-12 QS Kit technical specifications
Category	Details
Volume per PCR	25 µl
Matrix	BT5
Fluorescence labels	6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers	ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xlor Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis	Applied Biosystems GeneMapper ID, GeneMapperID-X or Genotyper Software

Applications

The Investigator Argus X-12 QS Kit enables simultaneous amplification of 12 X-chromosomal markers, and is highly suitable for:

Kinship and paternity testing, especially deficiency cases
Population genetics and anthropological studies
Analysis of stains with female traces on a male background

性能

说明书

Investigator Argus X-12 QS Kit Handbook

供应商

Qiagen