| 货号 | 6414S |
| 描述 | SignalSilence® 4E-BP1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit 4E-BP1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 参考文献 | Pause, A. et al. (1994) Nature 371, 762-7. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® 4E-BP1 siRNA I (+), using 4E-BP1 (53H11) Rabbit mAb #9644 and α-Tubulin (11H10) Rabbit mAb #2125. The 4E-BP1 (53H11) Rabbit mAb confirms silencing of 4E-BP1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. 使用4E-BP1 (53H11) Rabbit mAb兔单抗 #9644 and α-Tubulin (11H10) Rabbit mAb兔单抗 #2125，免疫印迹（Western blot）分析HeLa细胞中4E-BP1 (53H11)蛋白水平，细胞分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® 4E-BP1 siRNA I (+)。4E-BP1 (53H11) Rabbit mAb兔单抗确定4E-BP1基因沉默，然而α-Tubulin (11H10) Rabbit mAb兔单抗通常用于内参照。 |
