| 货号 | 6237S |
| 描述 | SignalSilence® Acetyl-CoA Carboxylase 1 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Acetyl-CoA Carboxylase 1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4). |
| 存放说明 | -20C |
| 参考文献 | Castle, J.C. et al. (2009) PLoS One 4, e4369. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86. Ha, J. et al. (1994) J Biol Chem 269, 22162-8. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I #6224 (+) or SignalSilence® Acetyl-CoA Carboxylase 1 siRNA II (+), using Acetyl-CoA Carboxylase 1 Antibody #4190 and α-Tubulin (11H10) Rabbit mAb #2125. The Acetyl-CoA Carboxylase 1 antibody confirms silencing of Acetyl-CoA Carboxylase 1 expression while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot检测HeLa细胞提取物，细胞用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I #6224 (+) 或SignalSilence® Acetyl-CoA Carboxylase 1 siRNA II (+)进行转染，检测抗体为Acetyl-CoA Carboxylase 1 Antibody #4190 (上) or α-Tubulin (11H10) Rabbit mAb兔单抗 #2125 (下)。Acetyl-CoA Carboxylase 1 Antibody验证了酰基辅酶A羟化酶1基因的沉默情况，α-Tubulin (11H10) Rabbit mAb兔单抗是内参对照。 |
