| 货号 | 6396S |
| 描述 | SignalSilence® Akt2 siRNA from Cell Signaling Technology allows the researcher to specifically inhibit Akt2 expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | Akt2 siRNA will inhibit human, mouse and rat Akt2 expression. |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).Small interfering RNA (siRNA) has been used to specifically silence Akt in CHO cells and 3T3-L1 adipocytes (18). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
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Western blot analysis of extracts from HeLa cells transfected with non-targeted (-) or Akt2 (+) siRNA. Akt2 was detected using Akt2 Antibody #2962, and p42 was detected using p42 MAPK Antibody #9108. The Akt2 Antibody confirms silencing of Akt2 expression, and the p42 MAPK Antibody is used to control for loading and specificity of the Akt2 siRNA. Western blot 分析HeLa细胞提取物，转染采用non-targeted (-) or Akt2 (+) siRNA.，Akt2采用Akt2 Antibody #2962检测， p42采用p42 MAPK Antibody #9108检测。Akt2 Antibody验证了Akt2表达的沉默， p42 MAPK Antibody作为上样和Akt2 siRN的特异性的对照。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. 荧光检测转染24小时后的活Hela细胞，采用SignalSilence® Control siRNA (Fluorescein Conjugate) #6201，显示近100%的转染效率。 |
