SignalSilence® ATF-2 siRNA II【科瑞奥博】

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SignalSilence® ATF-2 siRNA II

货号： 6443S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6443S
反应种属	Human
应用	TFN
使用方法	CST recommends transfection with 100 nM ATF-2 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

性能
供应商	CST
背景	The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
存放说明	-20C
参考文献	Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89. Gupta, S. et al. (1995) Science 267, 389-93. van Dam, H. et al. (1995) EMBO J 14, 1798-811. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

参考图片

Western blot analysis of extracts from ACHN cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATF-2 siRNA I #6433 (+) or SignalSilence® ATF-2 siRNA II (+), using ATF-2 (20F1) Rabbit mAb #9226 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The ATF-2 (20F1) Rabbit mAb confirms silencing of ATF-2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot 方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATF-2 siRNA I #6433 (+）和SignalSilence® ATF-2 siRNA II (+)的ACHN细胞，使用的抗体是ATF-2 (20F1) Rabbit mAb #9226 (上图) 和 α-Tubulin (11H10) Rabbit mAb #2125 (下图).ATF-2 (20F1) Rabbit mAb 确保ATF-2蛋白表达沉默，α-Tubulin (11H10) Rabbit mAb被当作对照载体来使用。