| 货号 | 6604S |
| 描述 | SignalSilence® Atg7 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Atg7 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). |
| 存放说明 | -20C |
| 参考文献 | Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. Mizushima, N. et al. (1998) Nature 395, 395-8. Suzuki, K. et al. (2001) EMBO J 20, 5971-81. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. Shintani, T. et al. (1999) EMBO J 18, 5234-41. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg7 siRNA I (+), using Atg7 Antibody #2631 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Atg7 Antibody confirms silencing of Atg7 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control. Western blot 分析HeLa细胞的提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® Atg7 siRNA I (+)转染，使用的抗体是Atg7 Antibody #2631（上图）或 β-Tubulin (9F3) Rabbit mAb 兔单抗#2128（下图）。在以 β-Tubulin (9F3) Rabbit mAb 兔单抗作为加样对照时，Atg7 Antibody 证实了Atg7表达的沉默。 |
