| 货号 | 6328S |
| 描述 | SignalSilence® ATM siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ATM expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.研究人员可以使用CST的SignalSilence® ATM siRNA I采用RNA干扰（一种通过传递外源性的双链RNA分子到细胞中从而能选择性的沉默基因表达的方法）技术特异性的抑制ATM蛋白表达。CST所有的SignalSilence® siRNA产品都经过了严格的内部测试并采用Western分析证明其可以有效降低靶蛋白的表达。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage (3,4).共济失调毛细血管扩张症突变蛋白激酶(ATM)是一种丝氨酸/苏氨酸激酶，能够调节细胞周期检验点和DNA修复(1)。 丝氨酸(1981位)自体磷酸化激活ATM的发生响应裸露的DNA双链断裂。 ATM激酶调节许多蛋白，涉及细胞周期检验点的控制、细胞凋亡和DNA修复。已知的ATM底物包括P53，CHK2，CHK1，CtIP，4E-BP1，BRCA1，RPA3，H2A.X，SMC1，FANCD2，Rad17，Artemis，Nbs1和PP1的I-2调节亚基(1,2)。ATM基因突变可导致共济失调毛细血管扩张症(AT)，一种常染色体隐性遗传病，该病特点是不协调的肌肉运动和神经退化。来自AT病人的细胞显示有缺陷的DNA损伤诱导检验点激活，对放射的敏感性和频率较高的染色体断裂(3,4)。 |
| 存放说明 | -20C |
| 参考文献 | Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8. Tang, X. et al. (2008) Mol Cell Biol 28, 2559-66. Shiloh, Y. (1997) Annu Rev Genet 31, 635-62. Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATM siRNA I (+) or SignalSilence® ATM siRNA II #6329 (+), using ATM (D2E2) Rabbit mAb #2873 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The ATM (D2E2) Rabbit mAb confirms silencing of ATM expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot方法分析细胞提取物。分别用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATM siRNA I (+)或 SignalSilence® ATM siRNA II #6329 (+)转染HeLa细胞。使用的抗体为ATM (D2E2) Rabbit mAb #2873 (上图)和α-Tubulin (11H10) Rabbit mAb #2125 (下图)。和对照抗体α-Tubulin (11H10) Rabbit mAb #2125相比，ATM (D2E2) Rabbit mAb #2873的检测结果证实ATM的表达被有效沉默。 |
