| 货号 | 6288S |
| 描述 | SignalSilence® ATR siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ATR expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.CST的SignalSilence® ATR siRNA I允许研究人员采用RNA干扰（一种通过传递外源性的双链RNA分子到细胞中从而能选择性的沉默基因表达的方法）技术特异性的抑制ATR蛋白表达。CST所有的SignalSilence® siRNA产品都经过了严格的内部测试并采用Western分析证明其可以有效降低靶蛋白的表达。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. While there have been no published reports of phosphorylation sites on ATR, Cell Signaling Technology has produced an antibody directed against phospho-ATR (Ser428) that demonstrates in vivo and UV-induced phosphorylation of this protein. This reagent could prove to be a valuable tool for monitoring ATR activation. Proline-directed phosphorylation sites like this one are often targeted by CDKs and MAPKs and can often dramatically affect protein conformation (4,5).共济失调毛细血管扩张症突变蛋白激酶(ATM)和共济失调毛细血管扩张症兼Rad3相关激酶(ATR)属于PI3激酶相关激酶(PIKK)家族成员，它们能够在毗邻一个谷氨酰胺的丝氨酸或者苏氨酸残基处磷酸化多重底物，以响应DNA损伤或者复制阻遏(1-3)。尽管ATR在细胞周期信号和DNA修复过程中发挥重要作用，对于其激活却知之甚少。虽然ATR磷酸化位点一直没有公开的报道，CST已经生产抗体针对磷酸化的ATR(Ser428)并证明了该蛋白在体和紫外诱导的磷酸化。该试剂将是监测ATR激活的一个很有价值的工具。类似这种的脯氨酸导向的磷酸化位点通常是CDKs和MAPKs的靶标，并往往能显著影响蛋白质的构象(4,5)。 |
| 存放说明 | -20C |
| 参考文献 | Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86. Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7. Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8. Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66. Smith, J. et al. (2010) Adv Cancer Res 108, 73-112. Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14. Liu, S. et al. (2011) Mol Cell 43, 192-202. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATR siRNA I (+) or SignalSilence® ATR siRNA II #6289 (+), using ATR Antibody #2790 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The ATR Antibody confirms silencing of ATR expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot方法分析HeLa细胞提取物。分别用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATR siRNA I (+) 或者SignalSilence® ATR siRNA II #6289 (+)转染HeLa细胞。使用的抗体为ATR Antibody #2790 (上图) or α-Tubulin (11H10) Rabbit mAb #2125 (下图)。和对照抗体α-Tubulin (11H10) Rabbit mAb #2125相比，ATR 抗体的检测结果证实ATR蛋白的表达被有效沉默。 | |
Western blot analysis of extracts from UV treated HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® ATR siRNA I (+), using Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb confirms reduction of ATR kinase activity, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot方法分析紫外处理的HeLa细胞提取物。分别用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或者 SignalSilence® ATR siRNA I (+)转染HeLa细胞。使用的抗体为Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909 (上图)和α-Tubulin (11H10) Rabbit mAb #2125 (下图)。和对照抗体α-Tubulin (11H10) Rabbit mAb #2125相比，Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909的检测结果证实ATR蛋白激酶的表达被有效沉默。 |
