| 货号 | 12763S |
| 描述 | SignalSilence® AUF1/hnRNP D siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit AUF1/hnRNP D expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® AUF1/hnRNP D siRNA I inhibits human, mouse, and monkey AUF1/hnRNP D expression. |
| 供应商 | CST |
| 背景 | AU-rich element RNA binding protein 1 (AUF1) is also known as heterogeneous ribonucleoprotein D (hnRNP D). AUF1 binds to the AU rich element (ARE) of target mRNA and regulates mRNA decay (1,2). It has a broad range of target genes including IL-1, IL-2, IL-3, Myc, TNF-α, and cyclin D1 (2). Binding of AUF1 to Myc mRNA also affects translation of Myc (3). Recent studies have provided evidence that AUF1 is also involved in the regulation of transcription. AUF1 binds to the promoters of various genes including complement receptor 2 (4), enkephalin (5), and α-fetoprotein (6). AUF1 also binds to the telomerase catalytic subunit Tert promoter and the G-rich telomeric repeat, thus regulating telomere maintenance and normal aging (7,8). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (9,10). All AUF1 isoforms shuttle between the nucleus and cytoplasm (11, 12). These isoforms have distinct localization and bind to different target mRNAs that contribute to the diversity of AUF1 function (2). |
| 存放说明 | -20C |
| 参考文献 | Brewer, G. (1991) Mol Cell Biol 11, 2460-6. Gratacós, F.M. and Brewer, G. (2010) Wiley Interdiscip Rev RNA 1, 457-73. Liao, B. et al. (2007) Nat Struct Mol Biol 14, 511-8. Tolnay, M. et al. (2000) Biochem J 348 Pt 1, 151-8. Dobi, A. et al. (2006) J Biol Chem 281, 28889-900. Jiao, R. et al. (2006) J Cell Biochem 98, 1257-70. Eversole, A. and Maizels, N. (2000) Mol Cell Biol 20, 5425-32. Pont, A.R. et al. (2012) Mol Cell 47, 5-15. Dempsey, L.A. et al. (1998) Genomics 49, 378-84. Wagner, B.J. et al. (1998) Genomics 48, 195-202. Zhang, W. et al. (1993) Mol Cell Biol 13, 7652-65. Sarkar, B. et al. (2003) J Biol Chem 278, 20700-7. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® AUF1/hnRNP D siRNA I (+), using AUF1/hnRNP D (D6O4F) Rabbit mAb #12382 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The AUF1/hnRNP D (D6O4F) Rabbit mAb confirms silencing of AUF1/hnRNP D expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot 方法检测293T细胞提取物。用100 nM SignalSilence® Control siRNA (未接合的) #6568 (-)或SignalSilence® AUF1/hnRNP D siRNA I (+)转染细胞。所用抗体为AUF1/hnRNP D (D6O4F) Rabbit mAb兔单抗 #12382 (上图) 或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下图)。AUF1/hnRNP D (D6O4F) Rabbit mAb兔单抗确定SP1表达的沉默效率，β-Actin (D6A8) 兔单抗作为上样量对照。 |
