| 货号 | 6471S |
| 描述 | SignalSilence® Bad siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bad expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
| 参考文献 | Yang, E. et al. (1995) Cell 80, 285-291. Zha, J. et al. (1996) Cell 87, 619-628. Datta, S.R. et al. (1997) Cell 91, 231-241. Peso, L. et al. (1997) Science 278, 687-689. Bonni, A. et al. (1999) Science 286, 1358-1362. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867. Harada, H. et al. (1999) Mol. Cell 3, 413-422. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557. Datta, S. et al. (2000) Mol. Cell 6, 41-51. Jin, Z. et al. (2004) J Biol Chem 279, 23837-44. |
