SignalSilence® Bax siRNA I【科瑞奥博】

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SignalSilence® Bax siRNA I

货号： 6321S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6321S
描述	SignalSilence® Bax siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bax expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
反应种属	Human
应用	TFN

性能
供应商	CST
背景	Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membranes permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).
存放说明	-20C
计算分子量	20
参考文献	Wei, M.C. et al. (2001) Science 292, 727-30. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6. Marzo, I. et al. (1998) Science 281, 2027-31. Bidere, N. et al. (2003) J. Biol. Chem. 278(33) , 31401-31411.

参考图片

Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.

荧光检测转染了SignalSilence® Control siRNA (荧光偶联) #6201 24小时后的 Hela 活细胞，图示表明了几乎100%的转染效率。

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bax siRNA I (+) or SignalSilence® Bax siRNA II #6514 (+), using Bax Antibody #2772 and α-Tubulin (11H10) Rabbit mAb #2125. The Bax Antibody confirms silencing of Bax expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bax siRNA.

Western免疫印迹分析转染了100 nM SignalSilence® Control siRNA (荧光偶联) #6201 (-)、SignalSilence® Bax siRNA I (+) 或SignalSilence® Bax siRNA II #6514 (+)的 Hela 细胞的细胞提取液，所用抗体为 Bax Antibody #2772和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。Bax Antibody证实对Bax表达的沉默，α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样量的对照和Bax siRNA的特异性验证。