| 货号 | 6516S |
| 描述 | SignalSilence® Bcl-2 siRNA II allows the researcher to specifically inhibit Bcl-2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® Bcl-2 siRNA II will inhibit human, mouse and rat Bcl-2 expression. |
| 供应商 | CST |
| 背景 | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7). |
| 存放说明 | -20C |
| 参考文献 | Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32. Deng, X. et al. (2001) J Biol Chem 276, 23681-8. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bcl-2 siRNA I #6441 (+), or SignalSilence® Bcl-2 siRNA II (+), using Bcl-2 (50E3) Rabbit mAb #2870 and α-Tubulin (11H10) Rabbit mAb #2125. Bcl-2 (50E3) Rabbit mAb confirms silencing of Bcl-2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bcl-2 siRNA. Western blot 免疫印迹分析转染了100 nM SignalSilence® Control siRNA (荧光偶联) #6201 (-),SignalSilence® Bcl-2 siRNA I #6441 (+)或SignalSilence® Bcl-2 siRNA II (+)的HeLa细胞的细胞提取液，所用抗体Bcl-2 (50E3) Rabbit mAb 兔单抗#2870和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。Bcl-2 (50E3) Rabbit mAb 兔单抗证实对Bcl-2表达的沉默,α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样量的对照，并验证Bcl-2 siRNA的特异性。 |
