| 货号 | 6362S |
| 描述 | SignalSilence® Bcl-xL siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bcl-xL expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5). |
| 存放说明 | -20C |
| 参考文献 | Adams, J.M. and Cory, S. (1998) Science 281, 1322-6. Minn, A.J. et al. (1999) EMBO J 18, 632-43. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9. Fan, M. et al. (2000) J Biol Chem 275, 29980-5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® BcL-xL siRNA I (+) or SignalSilence® Bcl-xL siRNA II #6363 (+), using Bcl-xL (54H6) Rabbit mAb #2764 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Bcl-xL (54H6) Rabbit mAb confirms silencing of Bcl-xL expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot分析转染了100 nM SignalSilence® Control siRNA (无荧光偶联) #6568 (-),SignalSilence® Bcl-xL siRNA I(+)或SignalSilence® Bcl-xL siRNA II #6363(+)的HeLa细胞的细胞提取液,用Bcl-xL (54H6) Rabbit mAb 兔单抗#2764(上图)和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125(下图)研究。Bcl-xL (54H6) Rabbit mAb 兔单抗证实对Bcl-xL表达的沉默,α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样量的对照。 |
