| 货号 | 6222S |
| 描述 | SignalSilence® Beclin-1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Beclin-1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). It is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response although responses to apoptosis appeared normal (7). Overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4). |
| 存放说明 | -20C |
| 参考文献 | Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. Kametaka, S. et al. (1998) J Biol Chem 273, 22284-91. Liang, X.H. et al. (1999) Nature 402, 672-6. Liang, X.H. et al. (1998) J Virol 72, 8586-96. Aita, V.M. et al. (1999) Genomics 59, 59-65. Liang, X.H. et al. (2001) Cancer Res 61, 3443-9. Yue, Z. et al. (2003) Proc Natl Acad Sci USA 100, 15077-82. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Beclin-1 siRNA I (+) or SignalSilence® Beclin-1 siRNA II #6246 (+), using Beclin-1 (D40C5) XP® Rabbit mAb #3495 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Beclin-1 (D40C5) XP® Rabbit mAb confirms silencing of Beclin-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Beclin-1 siRNA. Western blot 分析HeLa细胞的提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® Beclin-1 siRNA I (+) 或SignalSilence® Beclin-1 siRNA II #6246 (+)转染，使用的抗体是Beclin-1 (D40C5) XP® Rabbit mAb 兔单抗#3495上图）或 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 （下图）。以 α-Tubulin (11H10) Rabbit mAb 兔单抗作为加样和特异性的Beclin-1 siRNA对照，Beclin-1 (D40C5) XP® Rabbit mAb 兔单抗验证Beclin-1表达的沉默。 |
