| 货号 | 6461S |
| 描述 | SignalSilence® Bim siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bim expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity. |
| 存放说明 | -20C |
| 计算分子量 | 13 BimS, 16 BimL, 23 BimEL |
| 参考文献 | OConnor, L. et al. (1998) EMBO J 17, 384-95. Hsu, S.Y. et al. (1998) Mol Endocrinol 12, 1432-40. Bouillet, P. et al. (2002) Nature 415, 922-6. Whitfield, J. et al. (2001) Neuron 29, 629-43. Dijkers, P.F. et al. (2000) Curr Biol 10, 1201-4. Ley, R. et al. (2003) J Biol Chem 278, 18811-6. Puthalakath, H. et al. (1999) Mol Cell 3, 287-96. Lei, K. and Davis, R.J. (2003) Proc Natl Acad Sci U S A 100, 2432-7. Putcha, G.V. et al. (2003) Neuron 38, 899-914. Sunters, A. et al. (2003) J Biol Chem 278, 49795-805. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bim siRNA I (+) or SignalSilence® Bim siRNA II #6518 (+), using Bim (C34C5) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Bim (C34C5) rabbit mAb confirms silencing of Bim expression, while the α-tubulin (11H10) rabbit mAb is used to control for loading and specificity of Bim siRNA. Western blot 分析转染了100 nM SignalSilence® Control siRNA (荧光偶联) #6201 (-), SignalSilence® Bim siRNA I (+)或SignalSilence® Bim siRNA II #6518 (+)的HeLa细胞的细胞提取液，所用抗体是Bim (C34C5) Rabbit mAb 兔单抗和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。 Bim (C34C5) rabbit mAb 兔单抗证实对Bim表达的沉默,α-Tubulin (11H10) Rabbit mAb 兔单抗用作上样量的对照及验证Bim siRNA的特异性。 |
