| 货号 | 6442S |
| 描述 | SignalSilence® Bmi1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Bmi1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyl-transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit (7). Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells (3,4,8-10). |
| 存放说明 | -20C |
| 参考文献 | Boyer, L.A. et al. (2006) Nature 441, 349-53. Lee, T.I. et al. (2006) Cell 125, 301-13. Park, I.K. et al. (2003) Nature 423, 302-5. Molofsky, A.V. et al. (2003) Nature 425, 962-7. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67. Wang, H. et al. (2004) Nature 431, 873-8. Cao, R. et al. (2005) Mol Cell 20, 845-54. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7. Jacobs, J.J. et al. (1999) Nature 397, 164-8. Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Bmi1 siRNA I (+), using Bmi1 (D20B7) XP® Rabbit mAb #6964 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Bmi1 (D20B7) XP® Rabbit mAb confirms silencing of Bmi1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. 使用Bmi1 (D20B7) XP® Rabbit mAb #6964兔单抗 (上图)或α-Tubulin (11H10) Rabbit mAb #2125兔单抗 (下图)，免疫印迹（Western blot）分析HeLa细胞中Bmi1蛋白水平，细胞分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或SignalSilence® Bmi1 siRNA I (+)。Bmi1 (D20B7) XP® Rabbit mAb兔单抗确定Bmi1基因沉默，然而α-Tubulin (11H10) Rabbit mAb兔单抗通常作为内参。 |
