| 货号 | 6466S |
| 描述 | SignalSilence® Caspase-3 siRNA allows the researcher to specifically inhibit Caspase-3 expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
| 参考文献 | Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. Nicholson, D.W. et al. (1995) Nature 376, 37-43. Carlile, G. W. et al. (2004) Blood 103, 4310-4316. |
Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with non-targeted (control) siRNA or transfection with Caspase-3 siRNA (100 nM). Caspase-3 was detected using Caspase-3 Rabbit Monoclonal Antibody #9665, and p42 MAPK was detected using p42 MAPK Antibody #9108. Western blot方法检测HeLa细胞提取物，用空质粒、 非靶向 (对照) siRNA或100 nM Caspase-3 siRNA转染，48hr后检测；使用Caspase-3 Rabbit Monoclonal Antibody兔单抗 #9665 (上图) 检测Caspase-3，使用p42 MAPK Antibody #9108 (下图)检测p42 MAPK。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. SignalSilence® Control siRNA (Fluorescein Conjugate) #6201荧光检测转染24hr后的活HeLa细胞，结果显示了近100%的转染效率。 |
