| 货号 | 6520S |
| 描述 | SignalSilence® Caspase-3 siRNA II allows the researcher to specifically inhibit caspase-3 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | -20C |
| 参考文献 | Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. Nicholson, D.W. et al. (1995) Nature 376, 37-43. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Caspase-3 siRNA II (+), using Caspase-3 (8G10) Rabbit mAb #9665 and α-Tubulin (11H10) Rabbit mAb #2125. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of caspase-3 siRNA. Western blot方法检测HeLa细胞提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® Caspase-3 siRNA II (+)转染；使用Caspase-3 (8G10) Rabbit mAb 兔单抗#9665和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125检测。Caspase-3 (8G10) Rabbit mAb 兔单抗#9665确定Caspase-3表达的沉默，用α-Tubulin (11H10) Rabbit mAb 兔单抗作为内参对照。 |
