| 货号 | 12130S |
| 描述 | SignalSilence® Cbl-b siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Cbl-b expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | Cbl-b siRNA I inhibits human and monkey Cbl-b expression. |
| 供应商 | CST |
| 背景 | The Casitas B lineage lymphoma (Cbl) proteins (in mammals these are c-Cbl, Cbl-b, and Cbl-c) are a family of single subunit RING finger protein-ubiquitin E3 ligases that contain multiple protein interaction motifs (1). All Cbl proteins have a highly conserved N-terminal tyrosine kinase-binding (TKB) domain that mediates interactions between Cbl proteins and phosphorylated tyrosine residues on Cbl substrates. C-terminal to the RING finger, Cbl proteins have proline-rich domains that mediate interactions with SH3 domain-containing proteins. Phosphorylated tyrosine residues mediate interactions with SH2 domain-containing proteins such as the p85 subunit of PI3K. These protein-protein interaction motifs allow Cbl family proteins to function as adaptor proteins (2). This adaptor function contributes to the E3-dependent activities of Cbl proteins by targeting specific substrates for ubiquitination and degradation. The adaptor function also contributes to non-E3-dependent activities, such as the recruitment of proteins involved in receptor tyrosine kinase internalization, localization of Cbl proteins to specific subcellular compartments, and activation of discrete signaling pathways (1). |
| 存放说明 | -20C |
| 参考文献 | Schmidt, M.H. and Dikic, I. (2005) Nat Rev Mol Cell Biol 6, 907-18. Naramura, M. et al. (2002) Nat Immunol 3, 1192-9. Chiang, Y.J. et al. (2000) Nature 403, 216-20. Fang, D. and Liu, Y.C. (2001) Nat Immunol 2, 870-5. Heissmeyer, V. et al. (2004) Nat Immunol 5, 255-65. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Cbl-b siRNA I (+), or SignalSilence® Cbl-b siRNA II #12122 (+), using Cbl-b (D3C12) Rabbit mAb #9498 (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The Cbl-b (D3C12) Rabbit mAb confirms silencing of Cbl-b expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.Western blot检测转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)、SignalSilence® Cbl-b siRNA I (+)或SignalSilence® Cbl-b siRNA II #12122 (+)的293T细胞，采用Cbl-b (D3C12) Rabbit mAb #9498 (upper) 或GAPDH (D16H11) XP® Rabbit mAb #5174 (lower)。Cbl-b (D3C12) Rabbit mAb证实了Cbl-b的沉默表达，采用GAPDH (D16H11) XP® Rabbit mAb作为对照。 |
