| 货号 | 3500S |
| 描述 | SignalSilence® cdc2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit cdc2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.研究人员可以使用CST的SignalSilence® cdc2 siRNA I，采用RNA干扰（一种通过传递外源性的双链RNA分子到细胞中从而能选择性的沉默基因表达的方法）技术特异性的抑制cdc2 蛋白表达。CST所有的SignalSilence® siRNA产品都经过了严格的内部测试并采用Western分析证明其可以有效降低靶蛋白的表达。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂，cdc2的激活受多个步骤的调控，包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而，在细胞进入有丝分裂的过程中，激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位，抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。 |
| 存放说明 | -20C |
| 参考文献 | Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001. Norbury, C. et al. (1991) EMBO J 10, 3321-9. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71. Hunter, T. (1995) Cell 80, 225-36. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® cdc2 siRNA I (+) or SignalSilence® cdc2 siRNA II #3600 (+), using cdc2 (POH1) Mouse mAb #9116 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The cdc2 (POH1) Mouse mAb confirms silencing of cdc2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot方法检测HeLa细胞提取物：100nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)转染组，SignalSilence® cdc2 siRNA I (+)转染组 和 SignalSilence® cdc2 siRNA II #3600 (+)转染组，使用的抗体为cdc2 (POH1) Mouse mAb #9116 (上图) 或 α-Tubulin (11H10) Rabbit mAb #2125 (下图). 和对照抗体α-Tubulin (11H10) Rabbit mAb相比，cdc2 (POH1) Mouse mAb的检测结果证实 cdc2 的表达被有效沉默。 |
