| 货号 | 7417S |
| 描述 | SignalSilence® CDK2 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CDK2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.研究人员可以使用CST的SignalSilence® CDK2 siRNA II，采用RNA干扰（一种通过传递外源性的双链RNA分子到细胞中从而能选择性的沉默基因表达的方法）技术特异性的抑制CDK2 蛋白表达。CST所有的SignalSilence® siRNA产品都经过了严格的内部测试并采用Western分析证明其可以有效降低靶蛋白的表达。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).周期蛋白依赖性激酶2(p33CDK2)是细胞周期机制的重要组成部分。类似于p34cdc2，激酶活性的调节经由磷酸化状态以及周期蛋白亚基和CDK抑制剂之间的关系。磷酸化抑制通常发生在14位和15位苏氨酸(1)。CDK2-周期蛋白复合体的抑制也与p27 Kip1和 p21 Waf1/Cip1之间的关系有关(2)。CDK2复合体的磷酸化需要cdc25磷酸酶和160位苏氨酸磷酸化引(由CDK7和cyclin H组成的CAK复合体介导)起的14位和15位苏氨酸去磷酸化。CDK2/周期蛋白E激酶活性对于G1到S期的转换和Rb蛋白的磷酸化具有重要意义。在S期，活化的CDK2/周期蛋白A复合体支配和磷酸化E2F，而活化的CDK2复合体持续在G2期的胞核中存在(5)。 |
| 存放说明 | -20C |
| 参考文献 | Morgan, D.O. (1995) Nature 374, 131-4. Poon, R.Y. et al. (1996) J Biol Chem 271, 13283-91. Gu, Y. et al. (1992) EMBO J 11, 3995-4005. Fesquet, D. et al. (1993) EMBO J 12, 3111-21. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® CDK2 siRNA I #8618 (+) or SignalSilence® CDK2 siRNA II (+), using CDK2 (78B2) Rabbit mAb #2546 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The CDK2 (78B2) Rabbit mAb confirms silencing of CDK2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot方法检测HeLa细胞提取物：100nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)转染组，SignalSilence® CDK2 siRNA I #8618 (+)转染组 和 SignalSilence® CDK2 siRNA II (+)转染组，使用的抗体为CDK2 (78B2) Rabbit mAb #2546 (上图) 或 α-Tubulin (11H10) Rabbit mAb #2125 (下图)。和对照抗体α-Tubulin (11H10) Rabbit mAb相比，CDK2 (78B2) Rabbit mAb的检测结果证实CDK2 的表达被有效沉默。 |
