| 货号 | 6438S |
| 描述 | SignalSilence® CDK8 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CDK8 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.研究人员可以使用CST的SignalSilence® CDK8 siRNA I，采用RNA干扰（一种通过传递外源性的双链RNA分子到细胞中从而能选择性的沉默基因表达的方法）技术特异性的抑制CDK8蛋白表达。CST所有的SignalSilence® siRNA产品都经过了严格的内部测试并采用Western分析证明其可以有效降低靶蛋白的表达。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The mammalian Mediator Complex is a multi-subunit protein complex that couples specific transcriptional regulators to RNA polymerase II (Pol II) and the basal transcription machinery. Interactions between distinct Mediator subunits and transcription factors allow for specific gene regulation (reviewed in 1). Mediator complex interactions control various biological processes including insulin signaling (2), NF-κB-dependent signaling (3), stem cell pluripotency and self renewal (4,5), and proliferation of colon cancer cells (6,7). CDK8/Cyclin C, along with Med12 and Med13, constitutes a subcomplex within the Mediator Complex thought to act as a molecular switch, inhibiting Pol II recruitment and transcription initiation (8,9). Expression of CDK8 abrogates E2F-1-dependent inhibition of β-catenin activity in colon cancer cells (9). High levels of CDK8 coincide with high β-catenin-dependent transcription in colon cancer cells, and their proliferation can be inhibited by suppressing CDK8 expression (8).哺乳动物中介体复合物是一个多亚基的蛋白复合物，它能够偶联特异性的转录调节子至RNA聚合酶II (Pol II)和基础的转录子装置。不同的中介体亚基和转录因子的相互作用允许特异性的基因调节(回顾1)。中介体复合物相互作用控制各种生物学过程，包括胰岛素信号(2)，NF-κB-依赖的信号(3)，干细胞多能性和自我更新(4,5)和结肠癌细胞增殖(6,7)。CDK8/Cyclin C，连同Med12和Med13，在中介体复合物内部组成亚复合体，可以充当分子开关的角色，抑制Pol II招募和转录起始(8,9)。CDK8的表达能够废除结肠癌细胞中E2F-1-依赖的β-连环蛋白的活性(9)。高水平的CDK8与结肠癌细胞中高水平的β-连环蛋白依赖的转录相一致，而且其增殖可以通过抑制CDK8的表达被抑制(8)。 |
| 存放说明 | -20C |
| 参考文献 | Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63. Wang, W. et al. (2009) Dev Cell 16, 764-71. van Essen, D. et al. (2009) PLoS Biol 7, e73. Tutter, A.V. et al. (2009) J Biol Chem 284, 3709-18. Varelas, X. et al. (2008) Nat Cell Biol 10, 837-48. Firestein, R. et al. (2008) Nature 455, 547-51. Morris, E.J. et al. (2008) Nature 455, 552-6. Knuesel, M.T. et al. (2009) Mol Cell Biol 29, 650-61. Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51. |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® CDK8 siRNA I (+), using CDK8 (G398) Antibody #4101 and α-Tubulin (11H10) Rabbit mAb #2125. The CDK8 (G398) Antibody confirms silencing of CDK8 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot方法检测293细胞提取物：100nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)转染组，SignalSilence® CDK8 siRNA I (+)转染组，使用的抗体为CDK8 (G398) Antibody #4101 和 α-Tubulin (11H10) Rabbit mAb #2125。 和对照抗体α-Tubulin (11H10) Rabbit mAb相比，CDK8 (G398) Antibody的检测结果证实 CDK8 的表达被有效沉默。 |
