| 货号 | 6203S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM c-Jun siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12). |
| 存放说明 | -20C |
| 参考文献 | Jochum, W. et al. (2001) Oncogene 20, 2401-12. Davis, R.J. (2000) Cell 103, 239-52. Hilberg, F. et al. (1993) Nature 365, 179-81. Raivich, G. et al. (2004) Neuron 43, 57-67. Behrens, A. et al. (2002) EMBO J 21, 1782-90. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® c-Jun siRNA I (+) or SignalSilence® c-Jun siRNA II #6204 (+), using c-Jun (60A8) Rabbit mAb #9165 and α-Tubulin (11H10) Rabbit mAb #2125. The c-Jun (60A8) Rabbit mAb confirms silencing of c-Jun expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of c-Jun siRNA.western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® c-Jun siRNA I (+) 、SignalSilence® c-Jun siRNA II #6204 (+)的HeLa细胞，使用的抗体是c-Jun (60A8) Rabbit mAb #9165和α-Tubulin (11H10) Rabbit mAb #2125。用 c-Jun (60A8) Rabbit mAb 证实了c-Jun的沉默表达，而α-Tubulin (11H10) Rabbit mAb 被用于负载控制，以及c-Jun siRNA的特异性控制。 |
