| 货号 | 6204S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM c-Jun siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12). |
| 存放说明 | -20C |
| 参考文献 | Jochum, W. et al. (2001) Oncogene 20, 2401-12. Davis, R.J. (2000) Cell 103, 239-52. Hilberg, F. et al. (1993) Nature 365, 179-81. Raivich, G. et al. (2004) Neuron 43, 57-67. Behrens, A. et al. (2002) EMBO J 21, 1782-90. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® c-Jun siRNA I #6203 (+) or SignalSilence® c-Jun siRNA II (+), using c-Jun (60A8) Rabbit mAb #9165 and α-Tubulin (11H10) Rabbit mAb #2125. The c-Jun (60A8) Rabbit mAb confirms silencing of c-Jun expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of c-Jun siRNA.western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence® c-Jun siRNA I #6203 (+) 、SignalSilence® c-Jun siRNA II (+)的HeLa细胞，使用的抗体是c-Jun (60A8) Rabbit mAb #9165和α-Tubulin (11H10) Rabbit mAb #2125。用 c-Jun (60A8) Rabbit mAb 证实了c-Jun的沉默表达，而α-Tubulin (11H10) Rabbit mAb 被用于负载控制，以及c-Jun siRNA的特异性控制。 |
