| 货号 | 6389S |
| 描述 | SignalSilence® CK2α siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CK2α expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.研究人员可以使用CST的SignalSilence® CK2α siRNA I，采用RNA干扰（一种通过传递外源性的双链RNA分子到细胞中从而能选择性的沉默基因表达的方法）技术特异性的抑制CK2α蛋白表达。CST所有的SignalSilence® siRNA产品都经过了严格的内部测试并采用Western分析证明其可以有效降低靶蛋白的表达。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | CK2 (formerly called Casein Kinase II) is a highly conserved protein kinase with more than 300 substrates regulating cell growth, cell death, and cell survival. CK2 has been implicated in the response to UV irradiation-induced DNA damage, targeting XRCC1 (1) and BRCA1 (2) as well as regulating p53 tumor suppressor protein functions (3). Furthermore, CK2 plays a key role in NF-κB activation (4). UV irradiation stimulates CK2-mediated phosphorylation of several carboxy-terminal residues within IκBα, resulting in IκBα proteasomal degradation and the release and nuclear translocation of active NF-κB. CK2 is also dysregulated in many cancers (5) and neurodegenerative diseases such as Alzheimers and Parkinsons diseases (6). Structurally, CK2 is a multimeric protein complex consisting of two catalytic subunits (α or α) and two regulatory β subunits (7). CK2 is distributed ubiquitously and is apparently constitutively active (7). While cell cycle-dependent Ser-Pro phosphorylation sites have been identified on CK2α and CK2β, Tyr255 phosphorylation by the Src-related kinase c-Fgr seems to have the greatest effect on CK2α activity (8,9).CK2(以前称作酪蛋白激酶 II)是一种高度保守的具有300多个作用底物的蛋白激酶，能够调节细胞生长、细胞死亡和细胞存活。CK2在响应紫外照射诱导的DNA损伤中被发现，它能够靶向XRCC1 (1) 和BRCA1 (2)并能调节p53肿瘤抑制基因的功能(3)。此外，CK2在NF-κB的激活过程中发挥着关键的作用(4)。紫外照射能够刺激CK2介导的IκBα中几个羧基末端残基的磷酸化，引起IκBα的蛋白酶体降解和释放以及活化的NF-κB的核转移。CK2在许多肿瘤和神经退行性疾病如阿尔茨海默氏症和帕金森症中功能紊乱(6)。结构上讲，CK2是一个多聚化的蛋白复合体，由两个催化亚基(α or α)和两个调节亚基β组成(7)。CK2分布广泛，明显的组成活跃(7)。尽管CK2α 和CK2β上已鉴定出细胞周期依赖性的Ser-Pro磷酸化位点，Src-相关激酶 c-Fgr作用的255位酪氨酸磷酸化似乎对CK2α的活性有最大的影响（8,9)。 |
| 存放说明 | -20C |
| 参考文献 | Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24. OBrien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7. Trembley, J.H. et al. Biofactors 36, 187-95. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9. Litchfield, D.W. (2003) Biochem J 369, 1-15. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® CK2α siRNA I (+), using CK2α Antibody #2656 and α-Tubulin (11H10) Rabbit mAb #2125. The CK2α Antibody confirms silencing of CK2α expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot方法检测HeLa细胞提取物：100nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)转染组，SignalSilence® CK2α siRNA I (+)转染组，使用的抗体为CK2α Antibody #2656 和α-Tubulin (11H10) Rabbit mAb #2125。 和对照抗体α-Tubulin (11H10) Rabbit mAb相比，CK2α Antibody的检测结果证实CK2α的表达被有效沉默。 |
