| 货号 | 9400S |
| 描述 | SignalSilence® CK2α siRNA I (Rodent Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CK2α expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.Cell Signaling Technology (CST) 公司的SignalSilence® CK2α siRNA I (Rodent特异) 帮助研究人员使用RNA干扰技术特异性抑制ERCC1表达，该技术通过向细胞内导入双链RNA分子以选择性的抑制某些基因的表达。所有CST公司的SignalSilence® siRNA都经过了严格的内部测试，western分析显示能够能够降低目标蛋白的表达。Quality Control |
| 反应种属 | Mouse |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | CK2 (formerly called Casein Kinase II) is a highly conserved protein kinase with more than 300 substrates regulating cell growth, cell death, and cell survival. CK2 has been implicated in the response to UV irradiation-induced DNA damage, targeting XRCC1 (1) and BRCA1 (2) as well as regulating p53 tumor suppressor protein functions (3). Furthermore, CK2 plays a key role in NF-κB activation (4). UV irradiation stimulates CK2-mediated phosphorylation of several carboxy-terminal residues within IκBα, resulting in IκBα proteasomal degradation and the release and nuclear translocation of active NF-κB. CK2 is also dysregulated in many cancers (5) and neurodegenerative diseases such as Alzheimers and Parkinsons diseases (6). Structurally, CK2 is a multimeric protein complex consisting of two catalytic subunits (α or α) and two regulatory β subunits (7). CK2 is distributed ubiquitously and is apparently constitutively active (7). While cell cycle-dependent Ser-Pro phosphorylation sites have been identified on CK2α and CK2β, Tyr255 phosphorylation by the Src-related kinase c-Fgr seems to have the greatest effect on CK2α activity (8,9).CK2（也称为酪蛋白激酶II)）是一种有超过300个底物，高度保守的蛋白激酶，调控细胞生长，细胞死亡和细胞生存。CK2涉及到UV辐射引发的DNA损伤，靶向XRCC1(1)和BRCA1（2）以及调控p53抑癌蛋白发挥作用（3）。并且，CK2还能激活NF-κB（4）。UV照射会刺激CK2介导的IκBα羧基端多个残基的磷酸化，导致IκBα通过蛋白酶体降解以及NF-κB释放转位到细胞核发挥作用。CK2也在多种癌症疾病（5）以及神经障碍疾病例如阿尔兹海默氏病和帕金森病（6）中异常表达。从结构上看，CK2是一个包含有2个催化亚基（α或α’）和2个调控β亚基的多聚体蛋白复合物（7）。CK2表达广泛且持续激活（7）。在CK2α 和CK2β上发现了细胞周期依赖的丝氨酸-脯氨酸磷酸化位点，Src-相关激酶c-Fgr对CK2α的255位酪氨酸有很强的磷酸化作用（8,9）。 |
| 存放说明 | -20C |
| 参考文献 | Morales, J.C. and Carpenter, P.B. (2004) Sci Aging Knowledge Environ 2004, pe24. OBrien, K.A. et al. (1999) Biochem Biophys Res Commun 260, 658-64. Cox, M.L. and Meek, D.W. (2010) Cell Signal 22, 564-71. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7. Trembley, J.H. et al. Biofactors 36, 187-95. Perez, D.I. et al. (2010) Med Res Rev, Epub ahead of print. Bosc, D.G. et al. (1995) J Biol Chem 270, 25872-8. Donella-Deana, A. et al. (2003) Biochem J 372, 841-9. Litchfield, D.W. (2003) Biochem J 369, 1-15. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® CK2α siRNA I (Rodent Specific) (+), using CK2α Antibody #2656 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The CK2α Antibody confirms silencing of CK2α expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.转染了100 nM SignalSilence®对照siRNA (未标记) #6568 (-)或SignalSilence® CK2α siRNA I (Rodent Specific) (+)的NIH/3T3细胞提取物，使用CK2α 抗体 #2656 (上) 或 β-Actin (D6A8) Rabbit mAb #8457 (下)进行western blot检测。CK2α抗体用于确认CK2α的表达抑制，β-Actin (D6A8) Rabbit mAb用于上样对照。 |
