| 货号 | 6341S |
| 描述 | SignalSilence® c-Myc siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit c-Myc expression by RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 64-67 |
| 参考文献 | Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99. |
Western blot analysis of extracts from HeLa cells following mock transfection, transfection with nonspecific (control) siRNA or transfection with SignalSilence® c-Myc siRNA I, using c-Myc Antibody #9402 and p42 MAP Kinase (Erk2) Antibody #9108. The c-Myc antibody confirms silencing of c-Myc expression and the p42 MAPK antibody is used to control for loading and specificity of c-Myc siRNA. Western blot 检测HeLa细胞提取物，分别用空质粒、非特异性Control siRNA或SignalSilence® c-Myc siRNA I转染；使用c-Myc Antibody #9402或p42 MAP Kinase (Erk2) Antibody #9108检测。c-Myc antibody确定c-Myc表达的沉默，p42 MAPK antibody作为内参对照使用。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. 荧光检测SignalSilence® Control siRNA (Fluorescein Conjugate) #6201转染24小时后的HeLa细胞，观察到了近100%的转染效率。 |
