货号 | 6552S |
描述 | SignalSilence® c-Myc siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit c-Myc expression by RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
反应种属 | Human |
应用 | TFN |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® c-Myc siRNA II (+), using c-Myc Antibody #9402 and β-Actin (13E5) Rabbit mAb #4970. c-Myc Antibody confirms silencing of c-Myc expression and β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of c-Myc siRNA. Western blot方法检测HeLa细胞提取物, 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) 或SignalSilence® c-Myc siRNA II (+)转染;使用c-Myc Antibody #9402或β-Actin (13E5) Rabbit mAb兔单抗#4970检测。c-Myc antibody确定c-Myc表达的沉默,β-Actin (13E5) Rabbit mAb兔单抗作为内参对照使用。 |