| 货号 | 6268S |
| 描述 | SignalSilence® Cofilin siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit cofilin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6). |
| 存放说明 | -20C |
| 参考文献 | Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30. Condeelis, J. (2001) Trends Cell Biol 11, 288-93. Arber, S. et al. (1998) Nature 393, 805-9. Yang, N. et al. (1998) Nature 393, 809-12. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Cofilin siRNA I #6267 (+) or SignalSilence® Cofilin siRNA II (+), using Cofilin (D59) Antibody #3318 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Cofilin (D59) Antibody confirms silencing of cofilin expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. 使用Cofilin (D59) Antibody #3318 (上图)和α-Tubulin (11H10) Rabbit mAb #2125 (下图).，免疫印迹（Western blot）分析HeLa细胞中Cofilin蛋白水平，细胞分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-),SignalSilence® Cofilin siRNA I #6267 (+)和SignalSilence® Cofilin siRNA II (+)。Cofilin (D59) Antibody确定cofilin基因沉默，然而α-Tubulin (11H10) Rabbit mAb通常用于内参照。 |
